Our Next IVAN Research Round-table Workshop on
Thursday February 27, 2025, at 4:00 PM ET.
“Suppress or not to suppress?!… – CRAFT it ! ”
The topic of our round-table workshop February 27, 2025, at 4:00 PM ET USA will be on Suppress or not to suppress?!… – CRAFT it ! – Extracting essential biomarker signals directly from the full 1H NMR spectrum of existing serum samples.
We introduced a very efficient and powerful software tool, CRAFT (Complete Reduction to Amplitude-Frequency Table) to extract essential biomarker signals from horse serum 1H spectra, namely N+Me signals of lipoproteins (GlycA and GlycB) and a composite signal of phospho-glycoproteins (SPC), which are especially indicative of inflammatory conditions. It is an orthogonal approach to the sophisticated, yet somewhat costly experimental suppression techniques (DIRE, JEDI).
Our approach uses the full 1D spectrum (only water suppression applied). Partial overlap with other signals or broad background resonances can be efficiently handled. A special advantage of this approach that it can be used retrospectively on any full spectra recorded earlier.
We applied the CRAFT protocol to 20+20 horse serum samples of equal number of healthy and diseased subjects diagnosed with OCD (Osteochondritis Dissecans), respectively, as a case study. The integral ratios of the Gly and SPC signals clearly identify the diseased cohort with good validation in O-PLS-DA. Similar analyses of various datasets from earlier studies are also presented, a study of falcon blood samples to diagnosed with a respiratory disease, and that of samples from horses diagnosed with laminitis.
Panelists: Istvan Pelczer, James Chen, Christina Metaxas, Krish Krishnamurthy
As always, we encourage you to visit the IVAN web page http://ivanmr.com! You will find the links to past workshops and our recent IVAN ENC meeting on You Tube as well as the complete schedule of past and upcoming workshops.
Click on the link below to register and receive the meeting link! You are invited to a Zoom meeting. When: February 27, 2025 at 4:00 PM Eastern Time (US and Canada) Register in advance for this meeting: https://us02web.zoom.us/meeting/register/eVzbYxDmTgWclMbFfE6fzA
See You on February 27, 2025!
The 2025 Executive Committee of IVAN: Jon Webb (CEO of MRR and VP of Q One Americas), Dan Iverson, Krish Krishnamurthy, Dave Rice, Eric Schatzlein (MRR)