ML4NGP TRAINING SCHOOL on
NMR for Intrinsically
Disordered Proteins
03-07 FEBRUARY 2025
CEITEC Masaryk University, Brno, Czech Republic
1st ML4NGP Training School on NMR for Intrinsically
Disordered Proteins
03-07 February 2025│CEITEC-MU, Brno
│Welcome message
The COST Action ML4NGP is pleased to announce the 3rd Training School on NMR for Intrinsically
Disordered Proteins.
Nuclear Magnetic Resonance (NMR) is a versatile technique for studying biomolecules at atomic
resolution, including intrinsically disordered proteins (IDPs). A variety of NMR techniques have been
developed to obtain information about intermolecular contacts, conformational preferences,
protein dynamics, and interactions.
The aim of this Training School is to explore the origin of NMR data, the procedure of their analysis
and interpretation, including potential sources of bias and error. Throughout almost one week, the
participants will learn the principles and practicalities of NMR to better understand how data reflects
IDP behavior in near-native states. Discussions on NMR and other experimental techniques will
provide unique insights on the limitations of NMR for IDP research, creating opportunities for
advancing the field.
The Training School is targeted at bioinformatics and life sciences researchers with minimal prior
experience in NMR, emphasizing the fundamental understanding rather than conducting
measurements. The program combines theoretical sessions and interactive discussions with
hands-on practical sessions to ensure participants leave with a robust understanding of how NMR
data is used, the possible biases in analysis, and how these insights are advancing our knowledge
of non-globular proteins.
│Organization
Local Organizing Committee (CEITEC Masaryk University, Brno, Czech Republic)
Pavel Kadeřávek, Šárka Fajtlová, Dávid Tužinčin, Alice Laníková, Viktor Bartošík, Martin Černý, Viliam
Volko
Scientific Organizing Committee
Core Group ML4NGP
│Speakers and trainers
Pavel Kadeřávek (CEITEC-MU, Czech Republic)
Lukáš ŽÍdek (CEITEC-MU, Czech Republic)
Michele Vendruscolo (University of Cambridge, UK)
Jiří Mareš (University of Vienna, Austria)
Frans Mulder (Johannes Kepler University Linz, Austria)
Gyula Batta (University of Debrecen, Hungary)
Dennis Kurzbach (University of Vienna, Austria)
Marco Schiavina (University of Florence, Italy)
Fabien Ferrage (CNRS, France)
Martin Blackledge (Institut de Biologie Structurale, France)
Isabelle Landrieu (Lille University, France) – remote presentation
Enrico Luchinat (University of Florence, Italy)
Hugo van Ingen (Utrecht University, the Netherlands)
│Target Audience and Requirements
Exclusive for the working group members of the COST Action CA21160. To ensure a balanced and
diverse representation, participants will be selected by the organizing committee with attention to
gender balance, early career stage (PhD students and junior postdocs), and geographic diversity.
The number of spots available is limited to facilitate in-depth discussion and hands-on learning.
This Training School is targeted at PhD students and early career postdocs with (nearly) zero
experience in NMR who are interested in learning the basics of this technique to advance the study
of non-globular proteins, with a special focus on IDPs. Participants are expected to have prior
knowledge and basic training in Bioinformatics and Computational Biology or related sciences.
Knowledge of NMR is not a requirement.
│Registration
Registration desk will be open from 8:30 to 9:00 on February 03 in the lecture room (see below).
│Name Badges
Participants must wear their name badges when in the venue.
│Program
FEBRUARY 3, 2025
08:30-09:00 Registration (252 Lecture room – building D29)
09:00-09:05 Welcome and introduction (252 Lecture room – building D29)
09:05-09:45 Theory: NMR introduction | Lukáš Žídek
09:45-10:45 Theory: Chemical Shifts | Michele Vendruscolo
10:45-11:15 Coffee Break (211 Room – building C04)
11:15-12:50 Practice: NMR software | Pavel Kadeřávek (118 Room – building C04)
12:50-14:00 Lunch (211 Room – building C04)
14:00-15:00 Theory: Diffusion-Ordered NMR Spectroscopy | Gyula Batta (252 Lecture room – building
D29)
15:00-15:30 Coffee break (211 Room – building C04)
15:30-16:30 Practice: Chemical Shifts | Michele Vendruscolo (118 Room – building C04)
16:30-17:30 Practice: Diffusion-Ordered NMR Spectroscopy | Gyula Batta (118 Room – building C04)
17:30-18:00 Visit to NMR laboratory
18:00 Dinner (211 Room – building C04)
FEBRUARY 4, 2025
08:30-10:00 Theory: J couplings | Frans Mulder (252 Lecture room – building D29)
10:00-10:30 Coffee Break (211 Room – building C04)
10:30-12:30 Practice: J couplings | Frans Mulder (118 Room – building C04)
12:30-13:40 Lunch (211 Room – building C04)
13:40-15:30 Theory: NMR relaxation | Fabien Ferrage (252 Lecture room – building D29)
15:30-16:00 Coffee break (211 Room – building C04)
16:00-18:00 Practice: NMR relaxation | Fabien Ferrage (118 Room – building C04)
18:00 Dinner (211 Room – building C04)
FEBRUARY 5, 2025
08:30-09:30 Theory: Nuclear Overhauser Effect| Jiří Mareš (252 Lecture room – building D29)
9:30-10:00 Coffee Break (211 Room – building C04)
10:00-12:00 Practice: Nuclear Overhauser Effect| Jiří Mareš (118 Room – building C04)
12:00-13:10 Lunch (211 Room – building C04)
13:10-14:40 Theory: PRE, PRI, EPR, DNP | Dennis Kurzbach (252 Lecture room – building D29)
14:40-15:10 Coffee break (211 Room – building C04)
15:10-17:10 Practice: PRE, PRI, EPR, DNP | Dennis Kurzbach (118 Room – building C04)
18:00 Social Dinner
FEBRUARY 6, 2025
08:30-10:10 Theory: Interaction studies | Hugo van Ingen (252 Lecture room – building D29)
10:10-10:40 Coffee Break (211 Room – building C04)
10:40-12:40 Practice: Interaction studies | Hugo van Ingen (118 Room – building C04)
12:40-13:50 Lunch (211 Room – building C04)
13:50-15:30 Theory: DC and cross-relaxations | Marco Schiavina (252 Lecture room – building D29)
15:30-16:00 Coffee break (211 Room – building C04)
16:00-18:00 Practice: DC and cross-relaxations | Marco Schiavina (118 Room – building C04)
18:00 Dinner (211 Room – building C04)
FEBRUARY 7, 2025
08:30-09:45 Theory: Post-translational modifications | Isabelle Landrieu (252 Lecture room – building
D29)
9:45-10:45 Theory: In-cell NMR | Enrico Luchinat
10:45-11:15 Coffee Break (211 Room – building C04)
11:15-12:15 Practice: Post-translational modifications | Isabelle Landrieu (118 Room – building C04)
12:15-13:00 Practice: In-cell NMR | Enrico Luchinat
13:00-14:10 Lunch (211 Room – building C04)
14:10-17:50 Theory: IDPs ensembles | Martin Blackledge (252 Lecture room – building D29)
18:00 Dinner (211 Room – building C04)
│Social dinner
Social dinner will take place on Wednesday, 5th of February 2025, at 18:00 at Pivovarská Mendlák
restaurant, Mendlovo náměstí 158/20, 60300 Brno – Old Brno, Czechia:
https://en.mapy.cz/zakladni?q=pivovarska%20restaurace%20brno&source=firm&id=13632146&ds=2&x=16.5919870&y=49.1904349&z=17
│Event venue
CEITEC Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic (we recommend
entering via building E35 to get to the lecture hall – see map below)
Lecture room: 252 Lecture hall – UCB, Kamenice 5, Building D29, floor N02
https://www.muni.cz/en/map?q=252%20Posluch%C3%A1rna&id=BHA30N02052
Room for practical sessions: 118 Computer modeling laboratory – UCB, Kamenice 5, Building C04,
floor N01 https://www.muni.cz/en/map?q=C04%2F118&id=BHA05N01018
Coffee breaks, lunches, dinners: 211 Seminar room – UCB, Kamenice 5, Building C04, floor N02
https://www.muni.cz/en/map?q=C04%2F211&id=BHA05N02011
│Practical Information
TRANSPORT:
TAXI (some web pages are unfortunately only in the Czech language), some examples:
City Taxi: https://www.citytaxibrno.cz/en/
Top1 Taxi: https://www.top1taxi.cz/cenik/
Impuls Taxi: https://www.taxi-impuls.cz/cenik/
PUBLIC TRANSPORT
Public transport is available: https://www.dpmb.cz/en.
Public transport plan (daytime):
https://www.dpmb.cz/sites/default/files/downloads/2024/04/den_240427.pdf
Public transport plan (night):
Public transportation tickets are available in three formats: payment with a card (this is probably
the easiest option): https://pipniajed.cz/en.html; paper tickets (more information:
https://www.dpmb.cz/en/paper-tickets); or SMS tickets (more information:
https://www.dpmb.cz/en/mobile-phone-tickets).
LUNCHES AND DINNERS
Lunch and dinner can be ordered directly from the CEITEC campus through the online ordering
system until 15.1.2025. Different meal packages are available. Firstly, there is the possibility of buying
all the lunches and dinners for the whole week:
For those who are not planning to stay in Brno for the whole week (most likely speakers), it is
possible to order individual meals separately:
Monday, February 3:
lunch: https://is.muni.cz/obchod/baleni/366596?lang=en
dinner: https://is.muni.cz/obchod/baleni/366597?lang=en
Tuesday, February 4:
lunch: https://is.muni.cz/obchod/baleni/366598?lang=en
dinner: https://is.muni.cz/obchod/baleni/366599?lang=en
Wednesday, February 5:
lunch: https://is.muni.cz/obchod/baleni/366600?lang=en
social dinner at a restaurant: https://is.muni.cz/obchod/baleni/366601?lang=en
Thursday, February 6:
lunch: https://is.muni.cz/obchod/baleni/366602?lang=en
dinner: https://is.muni.cz/obchod/baleni/366603?lang=en
Friday, February 7:
lunch: https://is.muni.cz/obchod/baleni/366604?lang=en
dinner: https://is.muni.cz/obchod/baleni/366605?lang=en
Please let us know if you have any dietary restrictions so we can pass this information on to the
catering company.
ACCOMMODATION
Here are several suggestions for accommodation facilities and hotels (other possibilities can be
found as well):
Close to the event venue, within walking distance 3-5 minutes from CEITEC, is Campea Business
Apartments, Brno.
Close to the city center (approximately 20-25 minutes by public transport or 15 minutes by taxi):
Hotel International: https://www.hotelinternational.cz/en/
Hotel Continental: https://www.continentalbrno.cz/en
Hotel Passage: https://www.hotelpassage.eu/en/
Hotel Barceló: https://www.barcelo.com/en-ie/barcelo-brno-palace/
│Suggested readings
The practical session will be held on computers running the Linux system. Therefore, we ask those
who are not familiar with this system to learn the basic commands here:
https://www.geeksforgeeks.org/basic-linux-commands/
Acknowledgement of funding
This event is part of the activities of the COST Action ML4NGP, CA21160, which is supported
by COST (European Cooperation in Science and Technology).